RS rearrangement frequency as a marker of receptor editing in lupus and type 1 diabetes
نویسندگان
چکیده
Continued antibody gene rearrangement, termed receptor editing, is an important mechanism of central B cell tolerance that may be defective in some autoimmune individuals. We describe a quantitative assay for recombining sequence (RS) rearrangement that we use to estimate levels of antibody light chain receptor editing in various B cell populations. RS rearrangement is a recombination of a noncoding gene segment in the kappa antibody light chain locus. RS rearrangement levels are highest in the most highly edited B cells, and are inappropriately low in autoimmune mouse models of systemic lupus erythematosus (SLE) and type 1 diabetes (T1D), including those without overt disease. Low RS rearrangement levels are also observed in human subjects with SLE or T1D.
منابع مشابه
RS rearrangement frequency as a marker of receptor editing in lupus and type 1 diabetes Anil
The Rockefeller University Press $30.00 J. Exp. Med. Vol. 205 No. 13 2985-2994 www.jem.org/cgi/doi/10.1084/jem.20082053 2985 B cells undergo a random process of V(D)J recombination to generate the many distinct receptors needed to recognize a vast array of antigens. An inevitable consequence of this random process is the production of autoreactive B cells ( 1 ). An important mechanism for toler...
متن کاملRS rearrangement frequency as a marker of receptor editing in lupus and type 1 diabetes
The Rockefeller University Press $30.00 J. Exp. Med. Vol. 205 No. 13 2985-2994 www.jem.org/cgi/doi/10.1084/jem.20082053 2985 B cells undergo a random process of V(D)J recombination to generate the many distinct receptors needed to recognize a vast array of antigens. An inevitable consequence of this random process is the production of autoreactive B cells ( 1 ). An important mechanism for toler...
متن کاملRs Rearrangement: Observations and Implications from a Novel Assay of B Cell Tolerance
Antibody diversity is generated through the random recombination of immunoglobulin gene segments. As a consequence of this stochastic process, antibodies recognizing self-antigens can also be produced. Continued antibody gene rearrangement, termed receptor editing, is an important mechanism of central B cell tolerance that serves to alter the antibody specificity of developing B cells. Although...
متن کاملReceptor Editing Occurs Frequently during Normal B Cell Development By Marc
Allelic exclusion is established in development through a feedback mechanism in which the assembled immunoglobulin (Ig) suppresses further V(D)J rearrangement. But Ig expression sometimes fails to prevent further rearrangement. In autoantibody transgenic mice, reactivity of immature B cells with autoantigen can induce receptor editing, in which allelic exclusion is transiently prevented or reve...
متن کاملReceptor Editing Occurs Frequently during Normal B Cell Development
Allelic exclusion is established in development through a feedback mechanism in which the assembled immunoglobulin (Ig) suppresses further V(D)J rearrangement. But Ig expression sometimes fails to prevent further rearrangement. In autoantibody transgenic mice, reactivity of immature B cells with autoantigen can induce receptor editing, in which allelic exclusion is transiently prevented or reve...
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ورودعنوان ژورنال:
- The Journal of Experimental Medicine
دوره 205 شماره
صفحات -
تاریخ انتشار 2008